DIPLOMA IN TUBERCULOSIS TECHNICIAN (2 YEARS)
DIPLOMA IN TUBERCULOSIS TECHNICIAN (2 YEARS)
1st YEAR SYLLABUS
1. Basics of Human Anatomy & Physiology
Sub-Points
- Body organization & anatomical terms
- Respiratory system structure & functions
- Cardiovascular system basics
- Digestive, nervous & urinary systems (overview)
- Blood, immunity & lymphatic system
- Cell structure, tissues & basic histology
2. Fundamentals of Tuberculosis (TB)
Sub-Points
- Introduction to Tuberculosis
- Causative organism: Mycobacterium tuberculosis
- Types: Pulmonary & Extrapulmonary TB
- Epidemiology of TB in community
- Pathogenesis & stages of TB infection
- Mode of transmission & high-risk groups
3. Microbiology (General + Mycobacteriology)
Sub-Points
- Introduction to microbiology
- Classification of microorganisms
- Bacteria, viruses, fungi – basics
- Acid-fast bacilli (AFB)
- Ziehl–Neelsen (ZN) staining principles
- Culture media used for TB
- Safe handling of sputum samples
4. Laboratory Technology (Basics)
Sub-Points
- Laboratory equipment & instruments
- Microscope handling & maintenance
- Slide preparation
- Sample collection, labeling & preservation
- Infection control in laboratory
- Quality assurance in laboratory procedures
5. Public Health, Hygiene & Disease Prevention
Sub-Points
- Concept of public health
- Airborne diseases
- Personal hygiene & sanitation
- Community-level TB prevention methods
- Health education techniques
- Vaccination & BCG immunization basics
6. Practical / Laboratory Work
Sub-Points
- Sputum collection & smear preparation
- Basic microscopy practice
- Preparation of ZN stain
- Instrument cleaning & sterilization
- Record book maintenance
- Safety precautions & waste disposal
2nd YEAR SYLLABUS
1. Advanced Tuberculosis Studies
Sub-Points
- Detailed pulmonary TB pathology
- Extrapulmonary TB (lymph node, bone, CNS, GI, etc.)
- Drug-resistant TB (MDR-TB, XDR-TB) introduction
- TB in children & immunocompromised patients
- National TB programs (NTCP / RNTCP / NTEP concepts)
2. TB Diagnosis Techniques
Sub-Points
- Sputum smear microscopy (detailed)
- ZN staining – step-by-step
- Fluorescent microscopy (FM) basics
- CBNAAT / GeneXpert – overview
- LPA (Line Probe Assay) – introduction
- Interpretation of lab results
- Sample rejection criteria & reporting
3. Pathology & Clinical Aspects of TB
Sub-Points
- Symptoms of pulmonary TB
- Differential diagnosis of TB vs other respiratory diseases
- Radiology basics:
- Chest X-ray for TB (patterns)
- Mantoux test / Tuberculin skin test
- TB complications & prognosis
- Treatment categories (DOTS concept – theoretical)
4. Infection Control & Biosafety
Sub-Points
- Biosafety levels in TB laboratory
- Personal protective equipment (PPE)
- Lab waste management
- Airborne infection control practices
- Decontamination & sterilization
- Spill management
5. Health Education & TB Counseling
Sub-Points
- Counseling of TB patients
- Adherence & treatment support
- Community awareness programs
- Preventing stigma & social issues
- Recording & reporting tools
6. Clinical Practice / Internship & Viva
Sub-Points
- Posting in TB laboratory
- Collecting & processing sputum specimens
- Working with microscopy & staining
- Patient interaction & counseling basics
- Involvement in outreach TB programs
- Record keeping & register maintenance
- Final practical exam & viva

