PG DIPLOMA IN MEDICAL LABORATORY TECHNICIAN ( 2YEAR)
PG DIPLOMA IN MEDICAL LABORATORY TECHNICIAN (PGDMLT)
FIRST YEAR SYLLABUS
1. Human Anatomy
Study of the structure of the human body.
Topics
- Introduction to anatomy and medical terminology
- Cell structure and functions
- Types of tissues in the body
- Skeletal system
- Muscular system
- Digestive system
- Respiratory system
- Circulatory system
- Nervous system
- Urinary system
- Reproductive system
Practical
- Identification of bones and organs using charts/models
- Study of body systems with anatomical diagrams
- Demonstration using specimens or models
2. Human Physiology
Study of functions of body organs and systems.
Topics
- Blood composition and functions
- Cardiovascular system and heart function
- Respiratory system physiology
- Digestive system physiology
- Nervous system and reflex actions
- Endocrine glands and hormones
- Excretory system
- Reproductive physiology
Practical
- Blood pressure measurement
- Pulse rate counting
- Hemoglobin estimation
3. Basic Biochemistry
Study of chemical components and reactions in the body.
Topics
- Carbohydrates, proteins, and lipids
- Enzymes and enzyme action
- Vitamins and minerals
- Metabolism of carbohydrates
- Protein metabolism
- Lipid metabolism
- Acid–base balance
- Water and electrolyte balance
Practical
- Detection of glucose
- Detection of proteins
- Biochemical tests for food substances
4. Basic Microbiology
Study of microorganisms and their relation to diseases.
Topics
- Introduction to microbiology
- Bacterial cell structure
- Classification of microorganisms
- Sterilization and disinfection
- Culture media preparation
- Microscopy techniques
- Basic parasitology and mycology
Practical
- Gram staining technique
- Preparation of culture media
- Observation of bacteria under microscope
5. Clinical Pathology
Study of laboratory examination of body fluids.
Topics
- Urine examination (physical, chemical, microscopic)
- Stool examination
- Semen analysis
- Body fluid examination (CSF, pleural, ascitic)
- Pregnancy testing
Practical
- Urine routine examination
- Stool microscopy for parasites
- Semen analysis
SECOND YEAR SYLLABUS
1. Hematology
Study of blood, blood cells, and blood disorders.
Topics
- Composition and functions of blood
- Hemoglobin estimation methods
- RBC, WBC, and platelet counts
- ESR (Erythrocyte Sedimentation Rate)
- Peripheral blood smear examination
- Differential leukocyte count
- Blood coagulation tests
- Anemia and leukemia
Practical
- Hemoglobin estimation
- RBC and WBC counting
- Preparation and staining of blood smear
- Blood grouping and Rh typing
2. Clinical Biochemistry
Laboratory diagnosis using chemical analysis of blood and body fluids.
Topics
- Blood glucose estimation
- Diabetes diagnosis tests
- Liver function tests (LFT)
- Kidney function tests (KFT)
- Lipid profile
- Enzyme analysis
- Hormone testing
- Electrolyte analysis
Practical
- Blood glucose test
- Cholesterol and triglyceride estimation
- Urea and creatinine tests
- Bilirubin estimation
3. Medical Microbiology
Study of disease-causing microorganisms.
Topics
- Pathogenic bacteria
- Tuberculosis diagnosis
- Bacterial culture techniques
- Antibiotic sensitivity testing
- Basic virology
- Fungal infections (mycology)
- Parasitic infections
Practical
- Gram staining
- Acid-fast staining for tuberculosis
- Culture and identification of bacteria
- Antibiotic sensitivity testing
4. Immunology and Serology
Study of immune system and diagnostic tests based on antigen-antibody reactions.
Topics
- Immune system and immunity types
- Antigen-antibody reactions
- Widal test for typhoid
- HIV testing basics
- Hepatitis testing
- Rheumatoid factor test
- Rapid diagnostic tests
Practical
- Agglutination tests
- Rapid card tests
- ELISA demonstration
5. Histopathology and Cytology
Study of tissues and cells for disease diagnosis.
Topics
- Tissue fixation and preservation
- Tissue processing techniques
- Microtome section cutting
- Staining methods
- Identification of abnormal cells
- Pap smear examination
Practical
- Preparation of tissue slides
- Tissue staining techniques
- Microscopic examination of tissues
6. Blood Bank Technology
Study of blood collection, testing, and transfusion procedures.
Topics
- Blood groups and Rh factor
- Blood donor selection
- Blood storage and preservation
- Cross-matching techniques
- Blood transfusion reactions
- Blood bank management
Practical
- Blood grouping
- Cross-matching
- Blood storage techniques
7. Laboratory Management and Safety
Study of proper laboratory operation and safety.
Topics
- Laboratory equipment and instruments
- Quality control in laboratory testing
- Biomedical waste management
- Infection control practices
- Laboratory records and documentation
- Ethics in laboratory practice

