PG DIPLOMA IN MEDICAL LABORATORY TECHNICIAN ( 2YEAR)

PG DIPLOMA IN MEDICAL LABORATORY TECHNICIAN ( 2YEAR)

PG DIPLOMA IN MEDICAL LABORATORY TECHNICIAN (PGDMLT)

FIRST YEAR SYLLABUS

1. Human Anatomy

Study of the structure of the human body.

Topics

  • Introduction to anatomy and medical terminology
  • Cell structure and functions
  • Types of tissues in the body
  • Skeletal system
  • Muscular system
  • Digestive system
  • Respiratory system
  • Circulatory system
  • Nervous system
  • Urinary system
  • Reproductive system

Practical

  • Identification of bones and organs using charts/models
  • Study of body systems with anatomical diagrams
  • Demonstration using specimens or models

2. Human Physiology

Study of functions of body organs and systems.

Topics

  • Blood composition and functions
  • Cardiovascular system and heart function
  • Respiratory system physiology
  • Digestive system physiology
  • Nervous system and reflex actions
  • Endocrine glands and hormones
  • Excretory system
  • Reproductive physiology

Practical

  • Blood pressure measurement
  • Pulse rate counting
  • Hemoglobin estimation

3. Basic Biochemistry

Study of chemical components and reactions in the body.

Topics

  • Carbohydrates, proteins, and lipids
  • Enzymes and enzyme action
  • Vitamins and minerals
  • Metabolism of carbohydrates
  • Protein metabolism
  • Lipid metabolism
  • Acid–base balance
  • Water and electrolyte balance

Practical

  • Detection of glucose
  • Detection of proteins
  • Biochemical tests for food substances

4. Basic Microbiology

Study of microorganisms and their relation to diseases.

Topics

  • Introduction to microbiology
  • Bacterial cell structure
  • Classification of microorganisms
  • Sterilization and disinfection
  • Culture media preparation
  • Microscopy techniques
  • Basic parasitology and mycology

Practical

  • Gram staining technique
  • Preparation of culture media
  • Observation of bacteria under microscope

5. Clinical Pathology

Study of laboratory examination of body fluids.

Topics

  • Urine examination (physical, chemical, microscopic)
  • Stool examination
  • Semen analysis
  • Body fluid examination (CSF, pleural, ascitic)
  • Pregnancy testing

Practical

  • Urine routine examination
  • Stool microscopy for parasites
  • Semen analysis

SECOND YEAR SYLLABUS

1. Hematology

Study of blood, blood cells, and blood disorders.

Topics

  • Composition and functions of blood
  • Hemoglobin estimation methods
  • RBC, WBC, and platelet counts
  • ESR (Erythrocyte Sedimentation Rate)
  • Peripheral blood smear examination
  • Differential leukocyte count
  • Blood coagulation tests
  • Anemia and leukemia

Practical

  • Hemoglobin estimation
  • RBC and WBC counting
  • Preparation and staining of blood smear
  • Blood grouping and Rh typing

2. Clinical Biochemistry

Laboratory diagnosis using chemical analysis of blood and body fluids.

Topics

  • Blood glucose estimation
  • Diabetes diagnosis tests
  • Liver function tests (LFT)
  • Kidney function tests (KFT)
  • Lipid profile
  • Enzyme analysis
  • Hormone testing
  • Electrolyte analysis

Practical

  • Blood glucose test
  • Cholesterol and triglyceride estimation
  • Urea and creatinine tests
  • Bilirubin estimation

3. Medical Microbiology

Study of disease-causing microorganisms.

Topics

  • Pathogenic bacteria
  • Tuberculosis diagnosis
  • Bacterial culture techniques
  • Antibiotic sensitivity testing
  • Basic virology
  • Fungal infections (mycology)
  • Parasitic infections

Practical

  • Gram staining
  • Acid-fast staining for tuberculosis
  • Culture and identification of bacteria
  • Antibiotic sensitivity testing

4. Immunology and Serology

Study of immune system and diagnostic tests based on antigen-antibody reactions.

Topics

  • Immune system and immunity types
  • Antigen-antibody reactions
  • Widal test for typhoid
  • HIV testing basics
  • Hepatitis testing
  • Rheumatoid factor test
  • Rapid diagnostic tests

Practical

  • Agglutination tests
  • Rapid card tests
  • ELISA demonstration

5. Histopathology and Cytology

Study of tissues and cells for disease diagnosis.

Topics

  • Tissue fixation and preservation
  • Tissue processing techniques
  • Microtome section cutting
  • Staining methods
  • Identification of abnormal cells
  • Pap smear examination

Practical

  • Preparation of tissue slides
  • Tissue staining techniques
  • Microscopic examination of tissues

6. Blood Bank Technology

Study of blood collection, testing, and transfusion procedures.

Topics

  • Blood groups and Rh factor
  • Blood donor selection
  • Blood storage and preservation
  • Cross-matching techniques
  • Blood transfusion reactions
  • Blood bank management

Practical

  • Blood grouping
  • Cross-matching
  • Blood storage techniques

7. Laboratory Management and Safety

Study of proper laboratory operation and safety.

Topics

  • Laboratory equipment and instruments
  • Quality control in laboratory testing
  • Biomedical waste management
  • Infection control practices
  • Laboratory records and documentation
  • Ethics in laboratory practice

 

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